Characterization and mapping of human genes encoding zinc finger proteins

Journal Article · Thu Oct 31 23:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.21.9563· OSTI ID:5826543

Bray, P; Dawid, I B ^[1]; Lichter, P ^[2]; Thiesen, H J ^[3]; Ward, D C ^[4]

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1. National Institutes of Health, Bethesda, MD (United States)
2. Yale Univ., New Haven, CT (United States) Deutsches Krebsforschungszentrum, Heidelberg (West Germany)
3. Basel Inst. for Immunology (Switzerland)
4. Yale Univ., New Haven, CT (United States)

The zinc finger motif, exemplified by a segment of the Drosophila gap gene Krueppel, is a nucleic acid-binding domain present in many transcription factors. To investigate the gene family encoding this motif in the human genome, a placental genomic library was screened at moderate stringency with a degenerate oligodeoxynucleotide probe designed to hybridize to the His/Cys (H/C) link region between adjoining zinc fingers. Over 200 phage clones were obtained and are being sorted into groups by partial sequencing, cross-hybridization with oligodeoxynucleotide probes, and PCR amplification. Further, the genomic clones were cross-hybridized with a set of 30 zinc finger-encoding cDNAs (Kox1-Kox30) isolated from a human T-cell cDNA library. Four cDNAs (Kox4, Kox7, Kox12, and Kox15) were identified that match one or more genomic clones; these matches were confirmed by nucleotide sequence analysis. One or more clones from each locus were mapped onto human metaphase chromosomes by chromosomal in situ suppression hybridization with fluorescent probe detection. The authors mapped ZNF7-Kox4 to chromosome 8qter, ZNF19/Kox12 to 16q22, ZNF22/Kox15 to support the conclusion that the human genome contains many, probably several hundred, zinc finger genes with consensus H/C link regions.

• OSTI ID: 5826543
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:20; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
GENE AMPLIFICATION
GENES
GENETIC MAPPING
HETEROCHROMOSOMES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAPPING
METALLOPROTEINS
METALLOTHIONEIN
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS