Is DNA damage the signal for induction of thermal resistance. Induction by radiation in yeast

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576381· OSTI ID:6629045

Mitchel, R E.J.; Morrison, D P

Yeast, as well as higher eukaryotes, are induced to increase thermal resistnace (thermotolerance) by prior exposure to a heat stress. Prior exposure to an acute dose of either /sup 60/Co ..gamma.. or 254-nm ultraviolet radiation, at sublethal or fractionally lethal doses, is shown to cause a marked increase in the resistance of Saccharomyces cerevisiae to killing by heat. Partial induction by radiation followed by maximum induction by heat did not produce an additive response when compared to a maximum induction by heat alone, suggesting that the same process was induced by both heat and radiation. Irradiation with 254-nm uv light followed by an immediate, partial photoreversal of pyrimidine dimers with long-wavelength uv light resulted in a reduced level of resistance compared to cells not exposed to the photoreversal light, indicating that the cells specifically recognized pyrimidine dimers as a signal to increase their thermal resistance. Exposure to 254-nm uv or ionizing radiation induced thermal resistance in mutants defective in either excision repair (rad3, uv-sensitive) or recombinational repair (rad52, ..gamma..-sensitive), suggesting that recognition and repair of DNA damage by these systems are not a part of the signal which initiates an increase in resistance to heat. These data indicate that types of DNA damage as diverse as those produced by ionizing radiation and by ultraviolet light are recognized as a signal by the yeast cell to increase its thermal resistance.

• Research Organization: Chalk River Nuclear Lab., Ontario, Canada
• OSTI ID: 6629045
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 99:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550700 -- Microbiology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
560202 -- Thermal Effects-- Microorganisms-- (1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
COBALT 60
COBALT ISOTOPES
DNA REPAIR
ELECTROMAGNETIC RADIATION
EXTREME ULTRAVIOLET RADIATION
FUNGI
GAMMA RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MUTANTS
NUCLEI
ODD-ODD NUCLEI
PLANTS
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SURVIVAL CURVES
TEMPERATURE EFFECTS
ULTRAVIOLET RADIATION
YEARS LIVING RADIOISOTOPES
YEASTS