Immunological and viral aspects of radiation-induced tumors. Third annual report. [Mice, /sup 90/Sr, gamma radiation]

Technical Report · Wed Nov 30 04:00:00 EST 1977

OSTI ID:6623010

Reif, A E

We have attempted to induce bone sarcoma with FBJ virus in a total of 140 newborn mice. FBJ tumors have developed in CF1 mice, but not in mice of the other strains. Attempts to transplant FBJ tumors in CF1 mice have failed, since these mice are random bred. Because C57BL/6 mice are healthy, long-lived mice that have a low incidence of spontaneous tumors, we have used them for our initial work on strontium-90 carcinogenesis. The last of nine mice injected at the beginning of the study with 0.5 ..mu..c/gm body weight of strontium-90 has now died. Two of these mice developed bone sarcoma, of which one has been transplanted isogenically through eight transplant generations. Immunological experiments to test the antigenicity of this transplantable tumor are in progress. An extensive experiment in which 700 C57BL/6 female mice were divided into nineteen groups is now in its second year. Mice were given the following treatments: 0, 0.032, 0.01, 0.32, 1.0 and 2.0 ..mu..c/gm of strontium-90, 0, 0.032, 0.01, 0.32 and 1.0 ..mu..c/gm of strontium-90 plus 300 rad total body cesium-137 irradiation; and injection of active FBJ virus, inactive FBJ virus, and irradiated (10,000 rad) transplantable strontium-90-induced C57BL/6 osteosarcoma, all three modes of treatment given to groups that did and that did not receive prior injection of 1.0 ..mu..c/gm of strontium-90. To date, we have serially transplanted four osteogenic sarcomas that developed in these mice. We have also in serial passage a lymphoma that developed in a control C57BL/6 mouse that received no treatment. All these transplantable C57BL/6 tumor lines are intended for use in immunological experiments.

• Research Organization: Mallory Inst. of Pathology Research Foundation, Boston, MA (USA)
• OSTI ID: 6623010
• Report Number(s): TID-28748
• Country of Publication: United States
• Language: English

Related Subjects

560152 -- Radiation Effects on Animals-- Animals
560162* -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE TISSUES
CARCINOGENESIS
CONNECTIVE TISSUE
DISEASES
ELECTROMAGNETIC RADIATION
EVEN-EVEN NUCLEI
GAMMA RADIATION
IMMUNE REACTIONS
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
ISOTOPES
LYMPHOMAS
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NUCLEI
OSTEOSARCOMAS
PARASITES
PATHOGENESIS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RODENTS
SARCOMAS
SKELETAL DISEASES
STRONTIUM 90
STRONTIUM ISOTOPES
TISSUES
TRANSPLANTS
VERTEBRATES
VIRUSES
YEARS LIVING RADIOISOTOPES