Incorporation of radioiodotyrosines into proteins formed during cell-free translation. [/sup 125/I, rat liver]

Journal Article · Sat Mar 25 04:00:00 EST 1978 · J. Biol. Chem.; (United States)

OSTI ID:6620952

Scherberg, N H; Seo, H; Hynes, R

Radioiodination of transfer RNAs isolated from rat liver by a procedure chosen to preserve acylated amino acids resulted in the addition of iodine to bound tyrosine. The iodinated aminoacylated tRNA/sup Tyr/ was 10-fold purified from the total tRNA by rapid chromatography on benzoylated DEAE-cellulose. Nearly 90% of the isotope associated with the partially purified fraction was shown to occur in acylated monoiodotyrosine and diiodotyrosine, in approximately equal isotopic proportion. When the /sup 125/I-labeled aminoacyl-tRNAs were added to a rabbit reticulocyte lysate, iodotyrosines were incorporated into protein. Unlabeled aminoacylated liver tRNA reduced the incorporation of iodinated tyrosines as did purified, aminoacylated Escherichia coli tRNA/sup Tyr/. In a lysate coded by rat pituitary RNA the isotope was in part incorporated into a protein which was identical with rat prolactin or its prohormone, as determined by immunoprecipitation and electrophoresis. Following proteolytic digestion of the immunoprecipitate, 95% of the isotope was recovered in monoiodotyrosine and diiodotyrosine. The proportion of iodinated tyrosines incorporated into prolactin in the cell-free translation mixtures was increased 668-fold by pretreatment of the lysate with micrococcal nuclease.

• Research Organization: Univ. of Chicago
• OSTI ID: 6620952
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 253:6; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ESCHERICHIA COLI
GLANDS
GONADOTROPINS
HORMONES
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LIVER
LTH
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RNA
RODENTS
SYNTHESIS
TRANSFER RNA
TYROSINE
VERTEBRATES