Effects of insulin on messenger RNA activities in rat liver

Journal Article · Wed Feb 25 04:00:00 EST 1981 · J. Biol. Chem.; (United States)

OSTI ID:5301355

Hill, R E ^[1]; Lee, K L; Kenney, F T

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1. Univ. of Tennessee, Oak Ridge

Liver poly(A) RNA, isolated from adrenalectomized rats after insulin treatment, was translated in a nuclease-treated lysate of rabbit reticulocytes and quantitated for both total activity and the capacity to synthesize the insulin-inducible enzyme tyrosine amino-transferase. Analysis of the translated products from poly(A) RNA isolated 1 h after insulin treatment showed a 2.7-fold increase in activity of tyrosine aminotransferase mRNA. During the same interval, the capacity of poly(A) RNA to direct the synthesis of total protein in lysates also changed, showing a 30 to 40% increase in translational activity/unit of RNA. Increased translatability was apparent in all fractions of poly(A) RNA separated by centrifugation on sucrose gradients. Insulin thus appears to mediated a generalized changed in mRNAs leading to increased capacity for translation; induction of tyrosine aminotransferase may reflect unusual sensitivity to this effect of the hormone.

• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5301355
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 256:4; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINOTRANSFERASES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HORMONES
INSULIN
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
MESSENGER-RNA
NITROGEN TRANSFERASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RATS
REACTION KINETICS
RNA
RODENTS
SYNTHESIS
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES