Insulin enhances transcription of the tyrosine aminotransferase gene in rat liver

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5234618

Lee, K L; Isham, K R; Johnson, A; Kenney, F T

The mechanism of insulin-mediated induction of tyrosine aminotransferase in rat liver was investigated using a cloned cDNA probe. The level of the aminotransferase mRNA increases about four-fold following administration of the hormone. This induced mRNA accumulation does not require de novo protein synthesis. Nuclear runoff transcription assays in isolated liver nuclei demonstrate that insulin has a rapid and time-dependent stimulator effect on amiotransferase gene transcription. The magnitude of enhanced transcription fully accounts for the increased in the mRNA. The authors conclude that the induction of tyrosine aminotransferase in rat liver by insulin is a consequence of a selective increase in the rate of transcription of the aminotransferases gene.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5234618
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOTRANSFERASES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DNA
ENZYME INDUCTION
ENZYMES
GENE REGULATION
GENES
GLANDS
HORMONES
HYDROXY ACIDS
INSULIN
LIVER
MAMMALS
MESSENGER-RNA
NITROGEN TRANSFERASES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RATS
RNA
RODENTS
TRANSCRIPTION
TRANSFERASES
TYROSINE
VERTEBRATES