Association of eukaryotic aminoacyl-tRNA synthetases with polyribosomes

Journal Article · Thu Apr 10 04:00:00 EST 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5767700

Fedorov, A N; Al'zhanova, A T; Ovchinnikov, L P

After separation of a mitochondria-free extract of rabbit reticulocytes into a fraction of a ribosome-free extract and a fraction of mono- and polyribosomes the major portion of the aminoacyl-tRNA synthetase activity was found in the fraction of mono- and polyribosomes. All 15 of the aminoacyl-tRNA synthetases were found, although in somewhat different proportions, in these two fractions of the mitochondria-free extract of the reticulocytes. The amino-acyl-tRNA synthetases of the ribosome-free extract are present in two forms: an RNA-binding form and a form without affinity for high-molecular-weight RNAs. Aminoacyl-tRNA synthetases dissociated from complexes with polyribosomes are found only in RNA-binding form. All the aminoacyl-tRNA synthetases can be removed from these complexes by the addition of 16S rRNA from E. coli, poly(U), or tRNA from rabbit reticulocytes, which indicates that the aminoacyl-tRNA synthetases are in labile association with the RNA-component of the polyribosomes and that their interaction is rather nonspecific. After EDTA-induced dissociation of the polyribosomes aminoacyl-tRNA synthetase activity is found in a complex with both ribosomal subunits.

• Research Organization: Institute of Protein Research, Pushchino, USSR
• OSTI ID: 5767700
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:10; ISSN BIORA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ACYLATION
AMINO ACIDS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
COMPLEXES
COMPLEXOMETRY
ENZYME ACTIVITY
ENZYMES
ERYTHROCYTES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LIGASES
MAMMALS
MATERIALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PROTEINS
RABBITS
RADIOENZYMATIC ASSAY
RADIORECEPTOR ASSAY
RETICULOCYTES
RIBOSOMES
RNA
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES