Lethal and mutagenic effects of 4-nitroquinoline 1-oxide in uv-sensitive and wild-type CHO cell lines
Technical Report
·
OSTI ID:6620779
A wild-type Chinese hamster ovary cell line (CHO-9) and its uv-sensitive subclone (43-3B) have been used in characterizing the lethal and mutagenic effects of the chemical carcinogen, 4-nitroquinoline 1-oxide (4NQO). Cell survival as a function of 4NQO dose was determined for exponential and growth arrested populations of both cell lines. During exponential growth, 43-3B was 3.7 times more sensitive than CHO-9 (based on the ratio of the D/sub 0/ doses) to induced reproductive death by 4NQO. for both cell lines, cells treated during growth arrest survived at close to control values. Expression time for resistance at three loci, 6-thioguanine resistance (6TG/sup r/), ouabain resistance (OUA/sup R/) and resistance to diptheria toxin (DT/sup r/), was determined and was complete for all three markers by the eighth day post-treatment. Dose response curves for these markers measured on Day 8 were linear in CHO-9 over the range of doses tested. Preliminary results for induction of 6TG/sup r/ in 43-3B show the subclone to be hypermutable on a per dose basis but hypomutable at equal levels of survival (2.6 times less mutable compared at D/sub 0/). Studies of survival and mutation in synchronous cells were undertaken for CHO-9. A pronounced cell cycle effect was seen with lowest survival during late G1. Mutation induction was highest during G1; the degree and pattern of cycle variation depending on the locus examined.
- Research Organization:
- Lawrence Berkeley Lab., CA (USA)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6620779
- Report Number(s):
- LBL-14730; ON: DE83005858
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BODY
CELL CULTURES
CELL CYCLE
CELL PROLIFERATION
CHALCOGENIDES
DATA
DOSE-RESPONSE RELATIONSHIPS
DOSES
EXPERIMENTAL DATA
FEMALE GENITALS
GONADS
HAMSTERS
HETEROCYCLIC COMPOUNDS
INFORMATION
LETHAL DOSES
MAMMALS
MUTAGENESIS
NITRO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OVARIES
OXIDES
OXYGEN COMPOUNDS
PYRIDINES
QUINOLINES
RODENTS
SURVIVAL CURVES
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BODY
CELL CULTURES
CELL CYCLE
CELL PROLIFERATION
CHALCOGENIDES
DATA
DOSE-RESPONSE RELATIONSHIPS
DOSES
EXPERIMENTAL DATA
FEMALE GENITALS
GONADS
HAMSTERS
HETEROCYCLIC COMPOUNDS
INFORMATION
LETHAL DOSES
MAMMALS
MUTAGENESIS
NITRO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OVARIES
OXIDES
OXYGEN COMPOUNDS
PYRIDINES
QUINOLINES
RODENTS
SURVIVAL CURVES
VERTEBRATES