Cytotoxic and mutagenic properties of shale oil byproducts. II. Comparison of mutagenic effects at five genetic markers induced by retort process water plus near ultraviolet light in Chinese hamster ovary cells
Journal Article
·
· Environ. Mutagen.; (United States)
A Chinese hamster ovary (CHO) cell line heterozygous at the adenine phosphoribosyl transferase (APRT) locus was used for selection of induced mutants resistant to 8-azaadenine (8AA), 6-thioguanine (6TG), ouabain (OUA), emetine (EMT) and diphtheria toxin (DIP). The expression times necessary for optimizing the number of mutants recovered at the different loci have been determined using the known direct acting mutagen, far ultraviolet light (FUV), and a complex aqueous organic mixture (shale oil process water) activated with near ultraviolet light (NUV). The results indicate that optimal expression times following treatment with either mutagen was between 2 and 8 days. For CHO cells treated with shale oil process water and subsequently exposed to NUV a linear dose response for mutant induction was observed for all five genetic loci. At 10% surviving fraction of cells, between 35- and 130-fold increases above backgound mutation frequencies were observed for the various markers examined.
- Research Organization:
- Los Alamos National Lab., NM
- OSTI ID:
- 6606324
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 4; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mutagenesis testing with mammalian cells: validating and adapting a multiple-marker bioassay to activate and detect mutagens in crude samples for energy technology. Progress report, July 1975-September 1980
Cytotoxic and mutagenic properties of shale oil byproducts. I. Activation of retort process waters with near ultraviolet light
Cytotoxic and mutagenic properties of shale oil byproducts. I. Activation of retort process waters with near ultraviolet light
Technical Report
·
Tue Nov 04 23:00:00 EST 1980
·
OSTI ID:6807634
Cytotoxic and mutagenic properties of shale oil byproducts. I. Activation of retort process waters with near ultraviolet light
Journal Article
·
Wed Dec 31 23:00:00 EST 1980
· Environ. Mutagen.; (United States)
·
OSTI ID:5305916
Cytotoxic and mutagenic properties of shale oil byproducts. I. Activation of retort process waters with near ultraviolet light
Journal Article
·
Wed Dec 31 23:00:00 EST 1980
· Environ. Mutagen.; (United States)
·
OSTI ID:5447995
Related Subjects
04 OIL SHALES AND TAR SANDS
040700* -- Oil Shales & Tar Sands-- Health & Safety
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
CHO CELLS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
ENZYMES
FAR ULTRAVIOLET RADIATION
FOSSIL FUELS
FUELS
GENETIC EFFECTS
HYDROGEN COMPOUNDS
LIQUID WASTES
MINERAL OILS
MUTAGENESIS
MUTANTS
MUTATION FREQUENCY
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RADIATIONS
SHALE OIL
SPENT SHALES
TRANSFERASES
ULTRAVIOLET RADIATION
WASTE WATER
WASTES
WATER
040700* -- Oil Shales & Tar Sands-- Health & Safety
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
CHO CELLS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
ENZYMES
FAR ULTRAVIOLET RADIATION
FOSSIL FUELS
FUELS
GENETIC EFFECTS
HYDROGEN COMPOUNDS
LIQUID WASTES
MINERAL OILS
MUTAGENESIS
MUTANTS
MUTATION FREQUENCY
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RADIATIONS
SHALE OIL
SPENT SHALES
TRANSFERASES
ULTRAVIOLET RADIATION
WASTE WATER
WASTES
WATER