Cytotoxic and mutagenic properties of shale oil byproducts. I. Activation of retort process waters with near ultraviolet light

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860030305· OSTI ID:5305916

Strniste, G F; Chen, D J

Cultured Chinese hamster ovary (CHO) cells were exposed to dilutions of shale oil retort process waters obtained from three different retorting processes located in the Green River oil shale formations in the western part of the United States. Although the intensity of the response was dictated by the process water used, all induced a cytotoxic (reduction in colony-forming ability) and mutagenic (induced at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus) response in cells pretreated with dilutions of the waters and subsequent exposure to near ultraviolet light (NUV). Combinations of process water plus NUV yielded mutation frequencies as great as 50% that witnessed for the mutation frequency induced by the potent carcinogen far ultraviolet light. NUV alone was nontoxic and nonmutagenic at the doses of radiation used. Exposure of CHO cells in the dark to nontoxic dilutions of the process waters resulted in small but significant increases in 6-thioguanine resistent mutants (1-2 times background rates). The biological consequences resulting from the disposal of retort process waters into the delcate environment present in this oil shale region could be further complicated by this photoactivating process.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5305916
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 3; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CULTURES
CELL KILLING
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
FEMALE GENITALS
FOSSIL FUELS
FUELS
GONADS
HAMSTERS
HYDROGEN COMPOUNDS
LIQUID WASTES
MAMMALS
MINERAL OILS
MUTAGENESIS
NEAR ULTRAVIOLET RADIATION
OILS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
OVARIES
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RODENTS
SHALE OIL
ULTRAVIOLET RADIATION
VERTEBRATES
WASTE WATER
WASTES
WATER