Mitogenic effect of double-stranded RNA in human fibroblasts: role of autogenous interferon

Journal Article · Thu Jan 01 04:00:00 EST 1987 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041300107· OSTI ID:6618628

Vilcek, J; Kohase, M; Henriksen-DeStefano, D

The synthetic double-stranded RNA polyinosinate-polycytidylate (poly(l).poly(C)) was mitogenic in cultures of human foreskin fibroblasts, as demonstrated by a stimulation of /sup 3/H-thymidine incorporation and an increase in cell density. Poly(l).poly(C) is a potent inducer of interferon (IFN)-beta in human fibroblasts. Single-stranded poly(l) or poly(C) were not mitogenic in human fibroblasts and did not stimulate IFN production. Antiserum to interferon (IFN)-beta, added to poly(l).poly(C)-stimulated cultures in order to neutralize endogenously generated IFN, markedly amplified the mitogenic action. Under similar experimental conditions, antiserum to IFN-beta did not enhance the mitogenic action of epidermal growth factor (EGF). Dexamethasone enhanced the mitogenic action of poly(l).poly(C) in a manner similar to antiserum against IFN-beta. This effect of dexamethasone correlated with its marked inhibitory action on poly(l).poly(C)-stimulated IFN production. Together with the results of other related studies, these findings support the notion of an evolutionary link between the generation of a mitogenic signal and IFN induction. In addition, these results support the concept that autocrine secretion of IFN-beta can exert negative feedback control of cell proliferation.

• Research Organization: New York Univ. Medical Center, NY
• OSTI ID: 6618628
• Journal Information: J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 130:1; ISSN JCLLA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AZINES
BIOCHEMISTRY
CELL PROLIFERATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
FIBROBLASTS
GROWTH
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
INTERFERON
LABELLED COMPOUNDS
LYMPHOKINES
MITOGENS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RIBOSIDES
RNA
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS