Effects of 2-aminoanthracene and benzo(. cap alpha. )pyrene on interferon production in human foreskin fibroblasts
Conference
·
OSTI ID:5606097
Human foreskin fibroblast cell cultures (Flow 7000, FS-35) were treated with varying concentrations of 2-aminoanthracene or benzo(..cap alpha..)pyrene for a two-day period prior to interferon (IFN) induction by either poly I . poly C, or 'Vesicular' Stomatitis virus (VSV). IFN in test cell fluids was then assayed and compared to production in untreated cultures. Both cell lines produced similar amounts of beta-interferon in response to poly I . poly C simulus. However, Flow 7000 fibroblasts were extremely sensitive to benzo(..cap alpha..)pyrene, with concentrations as low as 1 ..mu..M treatment inhibiting 93% of IFN-..beta.. productivity. Treatment of FS-35 cells with ten times this concentration was required to inhibit 85% of IFN-..beta.. productivity. Both test chemicals inhibited IFN-..beta.. productivity in FS-35 fibroblasts to the same extent. It was also noted that FS-35 produced alpha-interferon in response to VSV challenge, but this production was vulnerable to treatment with both test chemicals. In all cases, observed levels of IFN inhibition were directly proportional to the concentration of the applied chemical. 1 fig., 4 tabs.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 5606097
- Report Number(s):
- BNL-40648; CONF-8711150-1; ON: DE88005602
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CULTURES
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
ENVIRONMENTAL EXPOSURE PATHWAY
FIBROBLASTS
GROWTH FACTORS
HYDROCARBONS
INTERFERON
LYMPHOKINES
MAMMALS
MAN
MICROORGANISMS
MITOGENS
ORGANIC COMPOUNDS
PARASITES
POLYCYCLIC AROMATIC AMINES
PRIMATES
PRODUCTION
PROTEINS
RESPONSE MODIFYING FACTORS
SOMATIC CELLS
VERTEBRATES
VIRUSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CULTURES
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
ENVIRONMENTAL EXPOSURE PATHWAY
FIBROBLASTS
GROWTH FACTORS
HYDROCARBONS
INTERFERON
LYMPHOKINES
MAMMALS
MAN
MICROORGANISMS
MITOGENS
ORGANIC COMPOUNDS
PARASITES
POLYCYCLIC AROMATIC AMINES
PRIMATES
PRODUCTION
PROTEINS
RESPONSE MODIFYING FACTORS
SOMATIC CELLS
VERTEBRATES
VIRUSES