Isolation of a novel human papillomavirus (type 51) from a cervical condyloma

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Journal of Virology; (USA)

OSTI ID:6617502

Nuovo, G J; Crum, C P; Levine, R U; Silverstein, S J ^[1]; De Villiers, E M ^[2]

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1. Columbia Univ., New York, NY (USA)
2. Deutsches Krebsforschungszentrum, Heidelberg (West Germany)

The authors cloned the DNA from a novel human papillomavirus (HPV) present in a cervical condyloma. When DNA from this isolate was hybridized at high stringency with HPV types 1 through 50 (HPV-1 through HPV-50), it showed weak homology with HPV-6 and -16 and stronger homology with HPV-26. A detailed restriction endonuclease map was prepared which showed marked differences from the maps for other HPVs that have been isolated from the female genital tract. Reassociation kinetic analysis revealed that HPV-26 and this new isolate were less than 10% homologous; hence, the new isolate is a noel strain of HPV. The approximate positions of the open reading frames of the new strain were surmised by hybridization with probes derived from individual open reading frames of HPV-16. In an analysis of 175 genital biopsies from patients with abnormal Papanicolaou smears, sequences hybridizing under highly stringent conditions to probes from this novel HPV type were found in 4.2, 6.1, and 2.4% of biopsies containing normal squamous epithelium, condylomata, and intraepithelial neoplasia, respectively. In addition, sequences homologous to probes from this novel isolate were detected in one of five cervical carcinomas examined.

• OSTI ID: 6617502
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 62:4; ISSN JOVIA; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOPSY
BODY
CARCINOMAS
CLONING
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
EPITHELIUM
FEMALE GENITALS
FEMALES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MICROORGANISMS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
TUMOR CELLS
UROGENITAL SYSTEM DISEASES
UTERUS
VERTEBRATES
VIRUSES
WOMEN