Comparison of peroxidase-labeled DNA probes with radioactive RNA probes for detection of human papillomaviruses by in situ hybridization in paraffin sections

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Modern Pathology; (USA)

OSTI ID:5546298

Park, J S; Kurman, R J; Kessis, T D; Shah, K V ^[1]

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1. Johns Hopkins Medical Institutions, Baltimore, MD (USA)

A study comparing in situ hybridization using nonradioactive DNA probes directly conjugated with horseradish peroxidase (HRP), and {sup 35}S-labeled antisense RNA probes for human papillomavirus (HPV) types 6/11, 16, and 18 was performed on formalin-fixed, paraffin-embedded tissue from 34 lesions of the cervix and vulva. These lesions included exophytic condylomas and intraepithelial and invasive neoplasms. HPV 6/11 was detected in two of four condylomata acuminata by both in situ techniques. HPV 16 was detected in 13 of 30 cases of intraepithelial and invasive neoplasms by both methods. Discordance between the two methods occurred in two instances. The radiolabeled probe but not the HRP probe detected HPV 16 in one case of cervical intraepithelial neoplasia (CIN 3), whereas the converse occurred in one case of vulvar intraepithelial neoplasia (VIN 3). HPV 18 was not detected in any of the specimens by either method. This study demonstrates that nonradioactive HRP-labeled probes for the detection of specific HPV types are as sensitive as the more laborious and potentially hazardous radioactive probes.

• OSTI ID: 5546298
• Journal Information: Modern Pathology; (USA), Journal Name: Modern Pathology; (USA) Vol. 4:1; ISSN 0893-3952; ISSN MODPE
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
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DNA HYBRIDIZATION
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FEMALE GENITALS
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OXIDOREDUCTASES
PEROXIDASES
RADIOASSAY
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RNA
SAMPLE PREPARATION
SULFUR 35
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