Transcriptional differences of the human papillomavirus type 16 genome between precancerous lesions and invasive carcinomas
Journal Article
·
· Journal of Virology; (USA)
OSTI ID:6522786
- Chiba Univ. (Japan)
Human papillomavirus type 16 (HPV16) genome DNA and its transcripts in biopsied cervical neoplasias were analyzed by simultaneous extraction of DNA and RNA from one biopsied sample. Southern blot analysis revealed that 5 of 20 cervical intraepithelial neoplasias (CINs) contained HPV16 DNAs existing primarily as episomes and two of seven invasive carcinomas harbored HPV16 genome sequences integrated into the host DNA. Northern (RNA) blot analysis showed that the HPV16 genome sequences were transcriptionally active in the five CINs, as well as in the two invasive carcinomas. The pattern of HPV16-specific transcripts in the CINs was uniform, and the major transcripts were 4.2, 2.2, 1.6, and 1.4 kilobases in size. However, the pattern of HPV16-specific transcripts in the invasive carcinomas was variable and different from that in CINs, suggesting that the alteration of transcriptional pattern might play a key role in the development of malignancy.
- OSTI ID:
- 6522786
- Journal Information:
- Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 62:3; ISSN 0022-538X; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma
Human papillomavirus types and recurrent cervical warts
Comparison of peroxidase-labeled DNA probes with radioactive RNA probes for detection of human papillomaviruses by in situ hybridization in paraffin sections
Journal Article
·
Sat Dec 31 23:00:00 EST 1988
· J. Virol.; (United States)
·
OSTI ID:6146244
Human papillomavirus types and recurrent cervical warts
Journal Article
·
Thu Mar 01 23:00:00 EST 1990
· JAMA, Journal of the American Medical Association; (USA)
·
OSTI ID:6699581
Comparison of peroxidase-labeled DNA probes with radioactive RNA probes for detection of human papillomaviruses by in situ hybridization in paraffin sections
Journal Article
·
Mon Dec 31 23:00:00 EST 1990
· Modern Pathology; (USA)
·
OSTI ID:5546298
Related Subjects
550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA HYBRIDIZATION
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MESSENGER-RNA
MICROORGANISMS
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RNA
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA HYBRIDIZATION
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MESSENGER-RNA
MICROORGANISMS
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RNA
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES
VIRUSES