Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma

Journal Article · Sun Jan 01 04:00:00 EST 1989 · J. Virol.; (United States)

OSTI ID:6146244

Woodworth, C D; Doniger, J; DiPaolo, J A

Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. Only transcripts from the intact E6 and E7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization. Recombinant HPV DNAs with low or no oncogenic potential for cervical cancer (HPV1a, -5, -6b, and -11) induced small G418-resistant colonies that senesced as did the nontransfected cells. These colonies contained only episomal virus DNA; therefore, integration of HPV sequences is important for immortalization of keratinocytes. This study suggests that the virus-encoded immortalization function contributes to the pathogenesis of cervical carcinoma.

• Research Organization: National Cancer Institute, Bethesda, MD (USA)
• OSTI ID: 6146244
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 63:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AUTORADIOGRAPHY
BODY
CARCINOMAS
CELL CONSTITUENTS
CELL TRANSFORMATIONS
DISEASES
DNA
DRUGS
FEMALE GENITALS
GENE RECOMBINATION
GENES
KERATIN
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
PATHOGENESIS
PLASMIDS
PROTEINS
SCLEROPROTEINS
SKIN
SOMATIC CELLS
TRANSCRIPTION
UTERUS
VIRUSES