Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: a preliminary communication

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Int. J. Gynecol. Pathol.; (United States)

DOI:https://doi.org/10.1097/00004347-198509000-00005· OSTI ID:6296268

Gupta, J; Gendelman, H E; Naghashfar, Z; Gupta, P; Rosenshein, N; Sawada, E; Woodruff, J D; Shah, K

Cervical Papanicolaou smears and paraffin sections of biopsy specimens obtained from women attending dysplasia clinics were examined for viral DNA sequences by in situ hybridization technique using TVS-labeled cloned recombinant DNA probes of human papillomavirus (HPV) types 6, 11, and 16. These and one unrelated DNA probe complementary to measles virus RNA were labeled by nick translation using either one or two TVS-labeled nucleotides. Paraffin sections and cervical smears were collected on pretreated slides, hybridized with the probes under stringent or nonstringent conditions for 50 h, and autoradiographed. Additional cervical specimens from the same women were examined for the presence of genus-specific papillomavirus capsid antigen by the immunoperoxidase technique. Preliminary results may be summarized as follows. The infecting virus could be identified in smears as well as in sections. Viral DNA sequences were detected only when there were condylomatous cells in the specimen and in only a proportion of the condylomatous cells. Even under stringent conditions, some specimens reacted with both HPV-6 and HPV-11. In some instances, the cells did not hybridize with any of the three probes even when duplicate specimens contained frankly condylomatous, capsid antigen-positive cells. In situ hybridization of Papanicolaou smears or of tissue sections is a practical method for diagnosis and follow-up of specific papillomavirus infection using routinely collected material.

• Research Organization: Johns Hopkins Medical Institutions, Baltimore, MD
• OSTI ID: 6296268
• Journal Information: Int. J. Gynecol. Pathol.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
RECOMBINANT DNA
HYBRIDIZATION
UTERUS
AUTORADIOGRAPHY
VIRAL DISEASES
DIAGNOSIS
DNA
PARAFFIN
SULFUR 35
VIRUSES
ALKANES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
FEMALE GENITALS
HYDROCARBONS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
PARASITES
RADIOISOTOPES
SULFUR ISOTOPES
WAXES
550601* - Medicine- Unsealed Radionuclides in Diagnostics