Herpes simplex virus in postradiation cervical smears. A morphologic and immunocytochemical study

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Acta Cytologica; (USA)

OSTI ID:6270927

Longatto Filho, A; Maeda, M Y; Oyafuso, M S; Kanamura, C T; Alves, V A ^[1]

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1. Adolfo Lutz Institute, Sao Paulo State Public Health Service (Brazil)

From January 1987 to August 1988, cytomorphologic criteria of both herpes simplex virus (HSV) and radiation effects were observed in Papanicolaou smears from 3 of 1,340 patients who had received radiotherapy for squamous cell carcinoma of the cervix. Avidin-biotin immunoperoxidase staining, using a rabbit IgG polyclonal HSV antibody, confirmed the presence of HSV antigen in those three postradiation smears. Both multinucleated molded cells and epithelial cells that lacked cytopathic effects were positive for HSV. Three other postradiation smears from these cases were similarly positive for HSV antigen; the one preradiation smear was negative. In situ hybridization and immunoperoxidase studies on sections from the preradiation biopsies were negative: severely altered neoplastic cells showed no reactivity. The absence of HSV markers in the preradiation specimens suggests that the HSV infections were secondary to the radiotherapy; further studies are needed to prove this association and to assess the possible mechanisms. These cases clearly indicate that the overlapping features of radiation and viral effects (such as multinucleation) may be present simultaneously.

• OSTI ID: 6270927
• Journal Information: Acta Cytologica; (USA), Journal Name: Acta Cytologica; (USA) Vol. 34:5; ISSN 0001-5547; ISSN ACYTA
• Country of Publication: United States
• Language: English

Related Subjects

560130 -- Radiation Effects on Microorganisms
560151* -- Radiation Effects on Animals-- Man
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARCINOMAS
DISEASES
FEMALE GENITALS
HERPES SIMPLEX
INFECTIOUS DISEASES
MEDICINE
MICROORGANISMS
NEOPLASMS
NUCLEAR MEDICINE
ORGANS
PARASITES
PATIENTS
RADIATION EFFECTS
RADIOLOGY
RADIOTHERAPY
SIDE EFFECTS
SKIN DISEASES
THERAPY
UTERUS
VIRAL DISEASES
VIRUSES