Induction of uterine cancer with inactivated herpes simplex virus, types 1 and 2

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Cancer (Philadelphia); (United States)

DOI:https://doi.org/10.1002/1097-0142(19811015)48:8<1783::AID-CNCR2820480815>3.0.CO;2-W· OSTI ID:5673027

Wentz, W B; Reagan, J W; Heggie, A D; Fu, Y S; Anthony, D D

A series of studies were performed to evaluate the oncogenic potential of inactivated herpes simplex viruses types 1 (HSV-1) and 2 (HSV-2) in the mouse cervix. HSV-1 or HSV-2 prepared in HEp-2 cell cultures and inactivated by exposure to formalin or ultraviolet light was applied to the mouse cervix for periods ranging from 20 to 90 weeks. Control mice were exposed for the same period to control fluids. Vaginal cytologic preparations from all animals were examined weekly to detect epithelial abnormalities. Animals were sacrificed and histopathological studies were carried out when cellular changes seen on vaginal smears resembled those indicative of premalignant or malignant changes as previously established in a similar model system using coal tar hydrocarbons. Other animals were exposed for periods up to 90 weeks, or until there was cellular evidence of invasive cancer. Cytologic and histologic materials were coded and evaluated without knowledge of whether they were from virus-exposed or control animals. Premalignant and malignant cervical lesions similar to those that occur in women were encountered in 78 to 90% of the virus-exposed animals. All controls were normal. Invasive cancer was detected in 24 to 60% of the animals and dysplasia was found in 18 to 66%. The yield of invasive cancer was twice as great after exposure to ultraviolet-inactivated HSV-2 as compared with formalin-inactivated virus. Various histologic grades of carcinoma of the cervix and endometrium were found. No primary lesions were found in the vagina or ovaries.

• Research Organization: Case Western Reserve University School of Medicine, Cleveland, Ohio
• OSTI ID: 5673027
• Journal Information: Cancer (Philadelphia); (United States), Journal Name: Cancer (Philadelphia); (United States) Vol. 48:8; ISSN CANCA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BODY
CYTOLOGY
DISEASES
ELECTROMAGNETIC RADIATION
FEMALE GENITALS
FORMALDEHYDE
HERPES SIMPLEX
INACTIVATION
INFECTIOUS DISEASES
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
RADIATION EFFECTS
RADIATIONS
RODENTS
SKIN DISEASES
ULTRAVIOLET RADIATION
UTERUS
VERTEBRATES
VIRAL DISEASES
VIRUSES