EFFECTS OF ACETONE, ULTRAVIOLET IRRADIATION AND FORMALIN ON WEST NILE VIRUS INFECTIVITY AND IMMUNOFLUORESCENT ANTIGENICITY

Journal Article · Fri Nov 01 04:00:00 EST 1963 · Proc. Soc. Exptl. Biol. Med.

DOI:https://doi.org/10.3181/00379727-114-28676· OSTI ID:4127598

Kundin, W D; Liu, C

An effort was made to ascertain whether infectious virus in thin sections could be inactivated without destroying either the antigenicity of the virus or the integrity of the tissue. Adjacent sections of the head from a suckling mouse dying of the Egypt 101 strain WN virus infection were exposed for varying lengths of time to the atmosphere (in the dark), to ultraviolet light, and to acetone (analytic grade) at room temperature, or to 10% buffered formalin at 4 deg C. Infectivity of West Nile Virus in frozen sections was inactivated by UV light in a matter of minutes while antigenicity persisted for several hours. Immersion of infected frozen sections in acetone inactivated 99% of virus infectivity in minutes yet antigenicity was not impaired for up to two weeks at room temperature. In our hands fixation by formalin was found to be unsatisfactory. (auth)

• Research Organization: Univ. of Kansas, Kansas City
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-003548
• OSTI ID: 4127598
• Journal Information: Proc. Soc. Exptl. Biol. Med., Journal Name: Proc. Soc. Exptl. Biol. Med. Vol. Vol: 114
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACETONE
ALDEHYDES
ANTIGENS
BIOLOGY AND MEDICINE
FORMIC ACID
IMMUNITY
INFECTIONS
MICE
RADIATION EFFECTS
TEMPERATURE
TOXICITY
ULTRAVIOLET RADIATION
VIRUSES