Immunogenicity of West Nile virus infectious DNA and its noninfectious derivatives

Journal Article · Wed Dec 20 04:00:00 EST 2006 · Virology

DOI:https://doi.org/10.1016/j.virol.2006.07.038· OSTI ID:20850580

Seregin, Alexey ^[1]; Nistler, Ryan ^[1]; Borisevich, Victoria ^[1]; Yamshchikov, Galina ^[1]; Chaporgina, Elena ^[1]; Kwok, Chun Wai ^[1]; Yamshchikov, Vladimir ^[1]

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1. Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045 (United States)

The exceptionally high virulence of the West Nile NY99 strain makes its suitability in the development of a live WN vaccine uncertain. The aim of this study is to investigate the immunogenicity of noninfectious virus derivatives carrying pseudolethal mutations, which preclude virion formation without affecting preceding steps of the viral infectious cycle. When administered using DNA immunization, such constructs initiate an infectious cycle but cannot lead to a viremia. While the magnitude of the immune response to a noninfectious replication-competent construct was lower than that of virus or infectious DNA, its overall quality and the protective effect were similar. In contrast, a nonreplicating construct of similar length induced only a marginally detectable immune response in the dose range used. Thus, replication-competent noninfectious constructs derived from infectious DNA may offer an advantageous combination of the safety of noninfectious formulations with the quality of the immune response characteristic of infectious vaccines.

• OSTI ID: 20850580
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1-2 Vol. 356; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBODIES
DNA
IMMUNE REACTIONS
MICE
MUTATIONS
VACCINES
VIRULENCE
VIRUSES