Physical mapping and YAC contig analysis of the region surrounding Xist on the mouse X chromosome

Journal Article · Mon Mar 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1108· OSTI ID:6616431

Heard, E; Simmler, M C; Rougeulle, C; Courtier, B; Avner, P ^[1]; Larin, Z ^[2]; Lehrach, H ^[3]

---

1. Institut Pasteur, Paris (France)
2. Univ. of Oxford (United Kingdom)
3. Imperial Cancer Research Fund Laboratories, London (United Kingdom)

The Xist sequence has been proposed as a potential candidate for the X-inactivation center based both on its localization within the candidate region for the X-inactivation center in man and mouse and on its unique pattern of expression from the inactive X chromosome. The authors have cloned 550 kb of DNA surrounding the mouse Xist sequence in contiguously overlapping YAC clones and have developed a long-range restriction map that spans almost 1 Mb of this region and includes this YAC contig. The detailed restriction map they have established provides a framework for the identification of expressed sequences other than Xist that may equally exhibit unusual expression characteristics associated with X inactivation. The presence of possible structural or methylation differences within this region between the active and inactive X chromosomes has been investigated through comparative analysis of male and female genomic DNA, and they report here the identification of certain CpG-containing restriction sites around Xist that have an interesting differential methylation status on the inactive and active X chromosomes. 43 refs., 7 figs., 2 tabs.

• OSTI ID: 6616431
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:3; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CHEMICAL REACTIONS
CHROMOSOMES
DNA
GENETIC MAPPING
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
INACTIVATION
MAMMALS
MAN
MAPPING
METHYLATION
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RODENTS
VERTEBRATES
X CHROMOSOME