Construction of a YAC contig spanning the Xq13.3 subband

Journal Article · Wed Mar 01 04:00:00 EST 1995 · Genomics

DOI:https://doi.org/10.1016/0888-7543(95)80089-5· OSTI ID:249970

Villard, L; Gecz, J; Colleaux, L ^[1]

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1. INSERM, Marseille (France); and others

The loci involved in several X-linked mental retardation syndromes have been linked to the pericentromeric region of the X chromosome long arm (Xq 12-q21). To isolate candidate genes for these diseases, the authors set up the construction of YAC contigs spanning this region. Two of these syndromes (the Juberg-Marsidi syndrome and the {alpha}-thalessemia mental retardation syndrome) have been recently linked, with high lod scores, to polymorphic probes previously assigned to Xq13.3. The authors therefore constructed a first YAC contig, encompassing this band, from DXS441 to PGK1. The physical map, deduced from the isolated clones, extends over 2.1 Mb of genomic DNA. Restriction analysis of the YAC contig allowed mapping precisely the loci previously assigned to that chromosomal region and to define their relative order. The validity of this physical map has been checked by comparing SfiI digest of the YACs to genomic fragments obtained with the same enzyme. A cDNA selection approach, already performed with a previous partial contig, has been extended to cover the whole region. 26 refs., 5 figs., 2 tabs.

• OSTI ID: 249970
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 26; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENETIC MAPPING
HUMAN X CHROMOSOME
MENTAL DISORDERS
PATHOGENESIS