An integrated YAC clone contig for the WAGR region on human chromosome 11p13-p14.1

Journal Article · Wed Nov 01 04:00:00 EST 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.0006· OSTI ID:273468

Gawin, B; Klamt, B; Koenig, A; Thaete, C ^[1]

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1. Biozentrum der Universitaet Wuerzburg (Germany); and others

The WAGR syndrome (Wilms tumor, aniridia, genito-urinary anomalies, and mental retardation) deletion region on chromosome 11p13 has been extensively characterized by deletion analysis and long-range restriction mapping. A dense probe set is available for this genomic region, which harbors a number of disease gene loci, some of which still are not cloned. The identification of candidates for these genes would be greatly facilitated by a complete gene map for this chromosomal segment. As an initial step toward this goal, we have isolated the entire region in 58 overlapping YAC clones. The contig spanning 8 Mb from RAG1 to KCNA4 has been assembled by STS and probe content mapping for 76 loci with an average spacing of about 100 kb. A subset of clones has been analyzed by PFG analysis to position these within the known physical map. Common microsatellite markers permit an alignment of the YAC contig with the genetic and radiation hybrid maps of chromosome 11. Ten known genes, some with much more refined map positions, are placed in the contig. The severalfold coverage of 11p13-p14.1 provides a reliable resource for the future development of a complete gene map of this region. 34 refs., 1 fig., 2 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 273468
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 30; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
CONTIGS
DESIGN
DNA-CLONING
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOMES
POLYMERASE CHAIN REACTION
PROBES