Physical mapping of 2000 kb of the mouse X chromosome in the vicinity of the Xist locus

Journal Article · Mon Mar 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1109· OSTI ID:6613940

Cooper, P; McCabe, V M; Rastan, S; Brockdorff, N ^[1]; Keer, J T; Hamvas, R M.J.; Brown, S D.M. ^[2]

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1. MRC Clinical Research Centre, Harrow (United Kingdom)
2. St. Mary's Hospital Medical School, London (United Kingdom)

A physical map encompassing approximately 2.0 megabases (Mb) in the region of the mouse X-inactivation center has been constructed. The map extends from the Gjb-1 locus to the Xist locus and demonstrates the order of probes inseparable by genetic analysis. The deduced locus order is as follows: Gjb-1, Ccg-1, DXCrc171, Rps4, Phka, DXCrc177, DXCrc318, Xist. Detailed physical mapping in the region between the Phka and Xist loci indicates the position of CpG-rich islands associated with the 5[prime] end of genes. The DXCrc177 and DXCrc318 loci, both defined by probes derived from linking clones, are associated with CpG-rich islands. The map provides a framework for the isolation of underlying sequences in the mouse X-inactivation center region. 27 refs., 4 figs., 2 tabs.

• OSTI ID: 6613940
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:3; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CHROMOSOMES
DNA
GENETIC MAPPING
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
INACTIVATION
MAMMALS
MAPPING
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RODENTS
VERTEBRATES
X CHROMOSOME