UV cross-linking identifies four polypeptides that require the TATA box to bind to the Drosophila hsp70 promoter
Journal Article
·
· Molecular and Cellular Biology; (USA)
- Washington Univ., St. Louis, MO (USA)
A protein fraction that requires the TATA sequence to bind to the hsp70 promoter has been partially purified from nuclear extracts of Drosophila embryos. This TATA factor produces a large DNase I footprint that extends from -44 to +35 on the promoter. A mutation that changes TATA to TATG interferes both with the binding of this complex and with the transcription of the hsp70 promoter in vitro, indicating that this interaction is important for transcriptional activity. Using a highly specific protein-DNA cross-linking assay, we have identified four polypeptides that require the TATA sequence to bind to the hsp70 promoter. Polypeptides of 26 and 42 kilodaltons are in intimate contact with the TATA sequence. Polypeptides of 150 and 60 kilodaltons interact within the region from +24 to +47 in a TATA-dependent manner. Both the extended footprint and the polypeptides identified by UV cross-linking indicate that the Drosophila TATA factor is a multicomponent complex.
- OSTI ID:
- 6611543
- Journal Information:
- Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:8; ISSN MCEBD; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
ARTHROPODS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL REACTIONS
CROSS-LINKING
DIPTERA
DNA
DNA-ASE
DROSOPHILA
ELECTROMAGNETIC RADIATION
EMBRYOS
ENZYMES
ESTERASES
FLIES
FRACTIONATION
FRUIT FLIES
HYDROLASES
INSECTS
INVERTEBRATES
KINETICS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHODIESTERASES
POLYMERIZATION
POLYPEPTIDES
PROTEINS
RADIATIONS
REACTION KINETICS
SEPARATION PROCESSES
TRANSCRIPTION
ULTRAVIOLET RADIATION
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
ARTHROPODS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL REACTIONS
CROSS-LINKING
DIPTERA
DNA
DNA-ASE
DROSOPHILA
ELECTROMAGNETIC RADIATION
EMBRYOS
ENZYMES
ESTERASES
FLIES
FRACTIONATION
FRUIT FLIES
HYDROLASES
INSECTS
INVERTEBRATES
KINETICS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHODIESTERASES
POLYMERIZATION
POLYPEPTIDES
PROTEINS
RADIATIONS
REACTION KINETICS
SEPARATION PROCESSES
TRANSCRIPTION
ULTRAVIOLET RADIATION