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TATA box-dependent protein-DNA interactions are detected on heat shock and histone gene promoters in nuclear extracts derived from Drosophila melanogaster embryos

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.8.8.3204· OSTI ID:6864342
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The authors monitored protein-DNA interactions that occur on the hsp26, hsp70, histone H3, and histone H4 promoters in nuclear extracts derived from frozen Drosophila melanogaster embryos. All four of these promoters were found to be transcribed in vitro at comparable levels by extracts from both heat-shocked and non-heat-shocked embryos. Factors were detected in both types of extracts that block exonuclease digestion from a downstream site at ca. +35 and -20 base pairs from the start of transcription of all four of these promoters. In addition, factors in extracts from heat-shocked embryos blocked exonuclease digestion at sites flanking the heat shock consensus sequences of hsp26 and hsp70. Competition experiments indicated that common factors cause the +35 and -20 barriers on all four promoters in both extracts. The formation of the barriers at +35 and -20 required a TATA box but did not appear to require specific sequences downstream of +7. The authors suggest that the factors responsible for the +35 and -20 barriers are components whose association with the promoter precedes transcriptional activation.
Research Organization:
Dept. of Biology, Washington Univ., St. Louis, MO (US)
OSTI ID:
6864342
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:8; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550400* -- Genetics
560200 -- Thermal Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BIOCHEMICAL REACTION KINETICS
CALMODULIN
DIPTERA
DNA
DROSOPHILA
FLIES
FRUIT FLIES
GENE AMPLIFICATION
GENES
HISTONES
INSECTS
INVERTEBRATES
KINETICS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
THERMAL SHOCK
TRANSCRIPTION