The glucocorticoid receptor binds to a sequence overlapping the TATA box of the human osteocalcin promoter: A potential mechanism for negative regulation
Journal Article
·
· Molecular and Cellular Biology; (United States)
- Huddinge Univ. Hospital (Sweden)
Expression of the human osteocalcin promoter is negatively regulated by glucocorticoids in vivo. In vitro DNase I and exonuclease III footprinting analysis showed binding of purified glucocorticoid receptor in close proximity to and overlapping with the TATA box of the osteocalcin gene. These results imply competition or interference with binding of the TATA box-binding transcription factor IID as a mechanism of repression of this gene by glucocorticoids. In support of this notion, point mutation analysis of the receptor binding site indicated that flanking nucleotides and not the TATA box motif per se were important for receptor interaction. Moreover, DNA binding competition assays showed specific binding of the receptor only to the TATA box region of the osteocalcin gene and not to the corresponding region of an immunoglobulin heavy-chain promoter.
- OSTI ID:
- 5116756
- Journal Information:
- Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:6; ISSN MCEBD; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL TISSUES
BODY
BONE TISSUES
CONNECTIVE TISSUE
CORTICOSTEROIDS
DNA SEQUENCING
DNA-ASE
ENZYMES
ESTERASES
GENE MUTATIONS
GENE REGULATION
GENE REPRESSORS
GLOBULINS
GLUCOCORTICOIDS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
IMMUNOGLOBULINS
KETONES
MEMBRANE PROTEINS
MUTATIONS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PREGNANES
PROTEINS
RECEPTORS
STEROID HORMONES
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
TRANSCRIPTION FACTORS
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL TISSUES
BODY
BONE TISSUES
CONNECTIVE TISSUE
CORTICOSTEROIDS
DNA SEQUENCING
DNA-ASE
ENZYMES
ESTERASES
GENE MUTATIONS
GENE REGULATION
GENE REPRESSORS
GLOBULINS
GLUCOCORTICOIDS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
IMMUNOGLOBULINS
KETONES
MEMBRANE PROTEINS
MUTATIONS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PREGNANES
PROTEINS
RECEPTORS
STEROID HORMONES
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
TRANSCRIPTION FACTORS