Production of high-quality edible protein from Candida. Yeast grown in continuous culture

Journal Article · Sun Jul 01 04:00:00 EDT 1979 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260210707· OSTI ID:6609521

Lawford, G R; Kligerman, A; Williams, T; Lawford, H G

Candida utilis NRRL Y-900 was grown in aerobic continuous culture with cane molasses as the source of the growth-limiting carbon. At 1% reducing sugar in the chemostat (10 liter working volume) feed medium, addition of Zn (25 mu M) to a minimal salts medium resulted in an increase in the biomass productivity of the chemostat from 1.7 to 2.6 g/liter/hr with a growth yield of 0.55 g dry biomass/g reducing sugar utilized at D max. On the average, the yeast biomass was 50-55% protein. At S R greater than 2% sugar, the biomass productivity was limited by the oxygen supply. With O/sub 2/-supplemented aeration (at reducing sugar equal to 4.2%) the maximum biomass productivity was 7.45 g/liter/hr. Aerobic ethanol production was not observed. A high quality undenatured protein fraction was isolated from the yeast homogenate by isoelectric precipitation at pH 4.5. Contaminating nucleic acid was removed as an insoluble complex by chelation with an organic cation (cetavlon). The final protein product contained about 3% RNA (DWB) and was suitable for use as a food additive.

• Research Organization: Weston Research Centre, 1047 Yonge Street, Toronto M4W 2L3, Ontario, Canada
• OSTI ID: 6609521
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 21:7; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AEROBIC CONDITIONS
CANDIDA
FOOD
FUNGI
MICROORGANISMS
MOLASSES
PLANTS
SINGLE CELL PROTEIN
YEASTS
YIELDS