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Continuous growth kinetics of Candida utilis in pineapple cannery effluent

Journal Article · · Biotechnol. Bioeng.; (United States)
Candida utilis was grown on a pineapple cannery effluent as the sole carbon and energy source in a chemostat at dilution rates between 0.10 and 0.62 h/sup -1/ to determine the growth kinetics. The principal sugars in the effluent were sucrose, glucose, and fructose. The cell yield coefficient on carbohydrate varied with dilution rate and a maximum value of 0.63 was observed at a dilution rate of 0.33 h/sup -1/. The steady-state concentrations of carbohydrate, reducing sugar, and chemical oxygen demand (COD) appeared to follow Monod saturation kinetics with increasing dilution rate, although none of the measured parameters represented a pure substrate. The maximum specific growth rate and reducing sugar saturation constant were 0.64 h/sup -1/ and 0.060 g/L, respectively. A maximum cell mass productivity of 2.3 g/L h was observed at a dilution rate of 0.51 h/sup -1/. At this dilution rate, only 68% of the COD was removed. A 95% COD removal was attained at a dilution rate of 0.10 h/sup -1/. Optimal yeast productivity and COD reduction occurred at a dilution rate of 0.33 h/sup -1/.
Research Organization:
Univ. of the Orange Free State, Bloemfontein, South Africa
OSTI ID:
6406645
Journal Information:
Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 26:7; ISSN BIBIA
Country of Publication:
United States
Language:
English