Comparison of (/sup 125/I)HIPDm and (/sup 125/I)iodoantipyrine in quantifying regional cerebral blood flow in rats

Journal Article · Tue Nov 01 04:00:00 EST 1988 · Stroke; (United States)

OSTI ID:6604461

Albright, Jr, R E; Friedman, A H; Fram, E K; Harbury, O L; Molter, B A; Skatoff, J H; Harris, C C; Coleman, R E; Drayer, B P

We determined regional cerebral blood flow (rCBF) using (/sup 125/I)HIPDm (N,N,N'-trimethyl-N'-(2-hydroxy-3-methyl-5-iodobenzyl)-1,3-propanediamin e) and (/sup 125/I)iodoantipyrine autoradiography under control and pathologic conditions (hypercapnia (acidosis), hypocapnia (alkalosis), and disrupted blood-brain barrier) conditions in 35 rats. In control rats, HIPDm rCBF (indicator fractionation method, n = 5) was lower than the corresponding IAP rCBF (diffusible indicator method, n = 4), most notably in the infratentorial regions and subcortical nuclei. In hypercapnia, rCBF increased by 100% and 37% in the HIPDm (n = 5) and IAP (n = 5) groups, respectively. In hypocapnia, IAP rCBF (n = 4) decreased 34% but HIPDm rCBF (n = 4) did not change. Following disruption of the blood-brain barrier by intracarotid infusion of mannitol in eight rats, both radiotracers (HIPDm n = 4, IAP n = 4) showed decreased rCBF to regions of disruption as defined by trypan blue extravasation. Our work indicates that modeling HIPDm uptake to quantify rCBF using the indicator fractionation method will underestimate blood flow and that HIPDm kinetics are influenced by compartmental pH dynamics that will limit the accuracy of this method in quantifying rCBF in pathologic conditions.

• Research Organization: Duke Univ. Medical Center, Durham, NC (USA)
• OSTI ID: 6604461
• Journal Information: Stroke; (United States), Journal Name: Stroke; (United States) Vol. 19:11; ISSN SJCCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIPYRETICS
ANTIPYRINE
AZOLES
BETA DECAY RADIOISOTOPES
BLOOD FLOW
BLOOD-BRAIN BARRIER
BODY
BRAIN
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
HYPERTENSION
HYPOTENSION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRAZOLES
PYRAZOLINES
RADIOISOTOPES
RADIOPHARMACEUTICALS
RATS
RODENTS
SYMPTOMS
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES