Metabolic and kinetic considerations in the use of (/sup 125/I)HIPDM for quantitative measurement of regional cerebral blood flow

Journal Article · Fri Mar 01 04:00:00 EST 1985 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1985.12· OSTI ID:5612765

Lucignani, G; Nehlig, A; Blasberg, R; Patlak, C S; Anderson, L; Fieschi, C; Fazio, F; Sokoloff, L

The metabolic degradation and the kinetics of the cerebral uptake of N,N,N'-trimethyl-N'-(2-hydroxy-3-methyl-5-(/sup 125/I)iodobenzyl)-1, 3-propanediamine ((/sup 125/I)HIPDM) have been studied in conscious, adult male Sprague-Dawley rats to determine its suitability as a tracer for the quantitative measurement of regional CBF (rCBF). rCBF was calculated by the indicator fractionation and the tissue equilibration methods in experiments of different durations up to 1 h. The values of rCBF obtained with (/sup 125/I)HIPDM were compared with those obtained in concurrent measurements with (/sup 14/C)iodoantipyrine in the same animals. Results of the experiments demonstrate that (/sup 125/I)HIPDM is an inadequate tracer for use with the indicator fractionation method and that any method that employs (/sup 125/I)HIPDM for the determination of rCBF must take into account its metabolic degradation, diffusion limitations, and bidirectional flux across the blood-brain barrier. With the tissue equilibration method, consistent determinations of rCBF may be possible with (/sup 125/I)HIPDM by measurement of the time course of its concentration in arterial blood, corrected for the presence of /sup 125/I-labeled metabolic products, and its concentration in the brain at any time up to 1 h after its administration. The method may be adapted to measure rCBF in humans by means of single-photon emission tomography with (/sup 123/I)HIPDM.

• Research Organization: National Institute of Mental Health, Bethesda, MD
• OSTI ID: 5612765
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Journal Name: J. Cereb. Blood Flow Metab.; (United States) Vol. 5:1; ISSN JCBMD
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BLOOD FLOW
BLOOD-BRAIN BARRIER
BODY
BRAIN
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CEREBRUM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATED AROMATIC HYDROCARBONS
INTERMEDIATE MASS NUCLEI
IODINATED AROMATIC HYDROCARBONS
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MALES
MAMMALS
METABOLISM
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANS
RADIOISOTOPES
RATS
RODENTS
VERTEBRATES