Comparison of fluorographic methods for the detection of /sup 35/S-labeled proteins in polyacrylamide gels

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(88)90204-7· OSTI ID:6602968

Perng, G G; Rulli, R D; Wilson, D L; Perry, G W

Eight different methods of fluorographic enhancement of sensitivity to /sup 35/S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5-diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of /sup 35/S after a 1-week exposure.

• Research Organization: Univ. of Miami, FL (USA)
• OSTI ID: 6602968
• Journal Information: Anal. Biochem.; (United States), Vol. 173:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
X-RAY FLUORESCENCE ANALYSIS
AUTORADIOGRAPHY
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
MEASURING METHODS
OXAZOLES
SENSITIVITY ANALYSIS
SULFUR 35
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL ANALYSIS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
ISOTOPES
LIGHT NUCLEI
NONDESTRUCTIVE ANALYSIS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RADIOISOTOPES
SULFUR ISOTOPES
X-RAY EMISSION ANALYSIS
550201* - Biochemistry- Tracer Techniques