Method for the typing of Clostridium difficile based on polyacrylamide gel electrophoresis of (/sup 35/S)methionine-labeled proteins
Journal Article
·
· J. Clin. Microbiol.; (United States)
OSTI ID:5687816
A typing method for Clostridium difficile based on the incorporation of (/sup 35/S)methionine into cellular proteins, their separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their visualization by autoradiography is described. On analysis of the radiolabeled-protein profiles, nine distinct groups were observed (A to E and W to Z). The method, which is simple, reproducible, and readily expandable, has been applied in epidemiological studies to demonstrate cross-infection and hospital acquisition of C. difficile.
- Research Organization:
- St. Bartholomew's Hospital Medical College, London, England
- OSTI ID:
- 5687816
- Journal Information:
- J. Clin. Microbiol.; (United States), Journal Name: J. Clin. Microbiol.; (United States) Vol. 1; ISSN JCMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AUTORADIOGRAPHY
BACTERIA
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BIOLOGY
CARBOXYLIC ACIDS
CLOSTRIDIUM
DAYS LIVING RADIOISOTOPES
DIARRHEA
DIGESTIVE SYSTEM DISEASES
DISEASES
DRUGS
ELECTROPHORESIS
EPIDEMIOLOGY
EVEN-ODD NUCLEI
FECES
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
METHIONINE
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
SYMPTOMS
WASTES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AUTORADIOGRAPHY
BACTERIA
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BIOLOGY
CARBOXYLIC ACIDS
CLOSTRIDIUM
DAYS LIVING RADIOISOTOPES
DIARRHEA
DIGESTIVE SYSTEM DISEASES
DISEASES
DRUGS
ELECTROPHORESIS
EPIDEMIOLOGY
EVEN-ODD NUCLEI
FECES
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
METHIONINE
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
SYMPTOMS
WASTES