Comparison of fluorographic methods for detecting radioactivity in polyacrylamide gels or on nitrocellulose filters
The commercial fluorographic enhancers, En3Hance or Amplify, were not as efficient as 2,5-diphenyloxazole (PPO) for detecting radioactively labeled proteins in polyacrylamide gels or on nitrocellulose filters. For most of the X-ray films tested, optimal preexposure was essential to obtain maximum sensitivity in fluorography or indirect autoradiography using intensifying screens. The best results were obtained with nitrocellulose by saturating the filters with PPO. The minimum levels of /sup 35/S//sup 14/C that could be detected on filters by autoradiography or fluorography in a 24-h exposure were 4 X 10(2) or 1 X 10(2) dpm cm-2 respectively. For /sup 3/H these levels were, respectively, 20 X 10(3) or 0.5 X 10(3) dpm cm-2.
- Research Organization:
- Univ. of Leeds, England
- OSTI ID:
- 5151531
- Journal Information:
- Anal. Biochem.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
AUTORADIOGRAPHY
AZOLES
CARBON 14 COMPOUNDS
COMPARATIVE EVALUATIONS
FILTERS
FILTRATION
FLUORESCENCE
NITROCELLULOSE
SULFUR 35
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELLULOSE ESTERS
CHEMICAL EXPLOSIVES
DAYS LIVING RADIOISOTOPES
ESTERS
EVEN-ODD NUCLEI
EXPLOSIVES
HETEROCYCLIC COMPOUNDS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LUMINESCENCE
NITRIC ACID ESTERS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SEPARATION PROCESSES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques