Use of the water-soluble fluor sodium salicylate for fluorographic detection of tritium in thin-layer chromatograms and nitrocellulose blots

Journal Article · Mon May 01 00:00:00 EDT 1989 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(89)90647-7· OSTI ID:5822215

Lucher, L A; Lego, T

We have determined that sodium salicylate, a water-soluble fluor which we use routinely for fluorography with polyacrylamide gels, is also useful for fluorography with thin-layer media. Detection of /sup 3/H-labeled material applied to thin-layer chromatography plates, or nitrocellulose membranes, can be enhanced up to 150-fold after treatment with an aqueous solution of 2 M sodium salicylate, while detection of /sup 35/S-labeled material is enhanced only about 2-fold. We demonstrate the utility of sodium salicylate fluorography in detecting 3H-labeled palmitic acid following thin-layer chromatography and /sup 3/H-labeled proteins following blotting to nitrocellulose.

• Research Organization: Illinois State Univ., Normal (USA)
• OSTI ID: 5822215
• Journal Information: Anal. Biochem.; (United States), Vol. 178:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HEXADECANOIC ACID
CHEMICAL ANALYSIS
PROTEINS
SALICYLIC ACID
FLUOROSCOPY
AQUEOUS SOLUTIONS
AUTORADIOGRAPHY
FLUORESCENCE
REAGENTS
SULFUR 35
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOMEDICAL RADIOGRAPHY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISPERSIONS
EVEN-ODD NUCLEI
HYDROXY ACIDS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LUMINESCENCE
MEDICINE
MIXTURES
MONOCARBOXYLIC ACIDS
NUCLEAR MEDICINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOISOTOPES
RADIOLOGY
SEPARATION PROCESSES
SOLUTIONS
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques