Zinc-binding proteins detected by protein blotting
Journal Article
·
· Anal. Biochem.; (United States)
The Western blotting technique was used for the detection of zinc-binding proteins. Proteins were separated electrophoretically on 15% polyacrylamide-sodium dodecyl sulfate minigels, the gels were soaked in a reduction buffer, and the proteins were transferred to nitrocellulose filters. Zinc-binding proteins were probed with radioactive zinc (/sup 65/Zn) and were detected by autoradiography. This technique allows the detection of as little as 20 to 100 pmol of zinc metalloproteins.
- Research Organization:
- Institut de Biologie Moleculaire et Cellulaire du CNRS, Strasbourg (France)
- OSTI ID:
- 6602942
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 172:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Detection of lipopolysaccharides in Polyacrylamide gels by transfer to nitrocellulose followed by immunoautoradiography with antibody and /sup 125/I-protein A: LPS blotting. [Gram negative bacteria]
Characterization of a zinc blotting technique: evidence that a retroviral gag protein binds zinc
Detection of DNA-protein binding in western blots by phosphorus-labeled and biotinylated DNA probes
Journal Article
·
Tue Feb 14 23:00:00 EST 1984
· Anal. Biochem.; (United States)
·
OSTI ID:5752168
Characterization of a zinc blotting technique: evidence that a retroviral gag protein binds zinc
Journal Article
·
Wed Jun 01 00:00:00 EDT 1988
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6922191
Detection of DNA-protein binding in western blots by phosphorus-labeled and biotinylated DNA probes
Journal Article
·
Thu Mar 31 23:00:00 EST 1988
· Biotechniques; (USA)
·
OSTI ID:6749623
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
METALLOPROTEINS
METALS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
ZINC
ZINC 65
ZINC ISOTOPES
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
METALLOPROTEINS
METALS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
ZINC
ZINC 65
ZINC ISOTOPES