Detection of lipopolysaccharides in Polyacrylamide gels by transfer to nitrocellulose followed by immunoautoradiography with antibody and /sup 125/I-protein A: LPS blotting. [Gram negative bacteria]
Journal Article
·
· Anal. Biochem.; (United States)
Lipopolysaccharides (LPS), which constitute the somatic (O) antigen of gram-negative bacteria, were used to demonstrate the procedure of LPS blotting involving the electrophoretic transfer of electrophoretically resolved LPS from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose filters. Immobilized LPS could then be immunoautoradiographically visualized in situ by reaction with specific anti-LPS antibody and subsequent binding of radioiodinated Staphylococcus protein A. LPS blotting is expected to provide an efficient and specific means of investigating the LPS (O) antigens of gram-negative bacteria. 18 references.
- Research Organization:
- Univ. of Toronto, Ontario
- OSTI ID:
- 5752168
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 137:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGEN-ANTIBODY REACTIONS
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CELLULOSE ESTERS
CHEMICAL EXPLOSIVES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ESTERS
EXPLOSIVES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIPIDS
LIPOPOLYSACCHARIDES
MICROORGANISMS
NITRIC ACID ESTERS
NITROCELLULOSE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC POLYMERS
POLYACRYLATES
POLYMERS
POLYSACCHARIDES
POLYVINYLS
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGEN-ANTIBODY REACTIONS
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CELLULOSE ESTERS
CHEMICAL EXPLOSIVES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ESTERS
EXPLOSIVES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIPIDS
LIPOPOLYSACCHARIDES
MICROORGANISMS
NITRIC ACID ESTERS
NITROCELLULOSE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC POLYMERS
POLYACRYLATES
POLYMERS
POLYSACCHARIDES
POLYVINYLS
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES