Changes in the antigenic and molecular structure of. gamma. -irradiated bacterial lipopolysaccharide (LPS)

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5072249

Csako, G; Suba, E A; Tsai, C M; Elin, R J

Ionization radiation is known to alter the biological properties of LPS. The author treated a highly purified LPS from E. coli in aqueous medium with /sup 60/Co-radiation. The changes in the antigenic and molecular structure of LPS were studied in double immunodiffusion/immunoelectrophoresis (rabbit antiserum) and sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Untreated LPS revealed two major antigenic components and, due to varying lengths of the O-polysaccharide side chain, a series of homopolymers (SDS-PAGE). High doses of ..gamma..-radiation destroyed all antigenic reactivities and all stainable bands on SDS-PAGE. However, lower doses of radiation were selective. Disappearance of the more radiation-sensitive, electrophoretically fast-migrating antigenic component paralleled elimination of the long O-side chain containing molecules. The relatively radiation-resistant, less anodic second antigenic component cross-reacted with LPS of another E. coli strain and corresponded to LPS molecules composed of R-core and lipid A (SDS-PAGE). These findings explain the in vivo loss of antibody protection from shock before non-specific resistance with ..gamma..-irradiation of LPS.

• Research Organization: National Institute of Health, Bethesda, MD
• OSTI ID: 5072249
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOHYDRATES
COBALT 60
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ESCHERICHIA COLI
GAMMA RADIATION
IMMUNE REACTIONS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIPIDS
LIPOPOLYSACCHARIDES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MOLECULAR STRUCTURE
MOLECULES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SACCHARIDES
YEARS LIVING RADIOISOTOPES