Changes in the antigenic and molecular structure of. gamma. -irradiated bacterial lipopolysaccharide (LPS)
Ionization radiation is known to alter the biological properties of LPS. The author treated a highly purified LPS from E. coli in aqueous medium with /sup 60/Co-radiation. The changes in the antigenic and molecular structure of LPS were studied in double immunodiffusion/immunoelectrophoresis (rabbit antiserum) and sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Untreated LPS revealed two major antigenic components and, due to varying lengths of the O-polysaccharide side chain, a series of homopolymers (SDS-PAGE). High doses of ..gamma..-radiation destroyed all antigenic reactivities and all stainable bands on SDS-PAGE. However, lower doses of radiation were selective. Disappearance of the more radiation-sensitive, electrophoretically fast-migrating antigenic component paralleled elimination of the long O-side chain containing molecules. The relatively radiation-resistant, less anodic second antigenic component cross-reacted with LPS of another E. coli strain and corresponded to LPS molecules composed of R-core and lipid A (SDS-PAGE). These findings explain the in vivo loss of antibody protection from shock before non-specific resistance with ..gamma..-irradiation of LPS.
- Research Organization:
- National Institute of Health, Bethesda, MD
- OSTI ID:
- 5072249
- Report Number(s):
- CONF-8604222-; TRN: 86-037067
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPOPOLYSACCHARIDES
BIOLOGICAL RADIATION EFFECTS
MOLECULAR STRUCTURE
ANTIGENS
COBALT 60
ELECTROPHORESIS
ESCHERICHIA COLI
GAMMA RADIATION
IMMUNE REACTIONS
MOLECULES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOHYDRATES
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIPIDS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SACCHARIDES
YEARS LIVING RADIOISOTOPES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)