Dose-dependent changes in the antigenicity of bacterial endotoxin exposed to ionizing radiation. Report No. 2, 1986-1987
Technical Report
·
OSTI ID:6074336
The antigenic properties of the highly purified US reference standard endotoxin (RSE) exposed to varying doses of ionizing radiation were studied with double immuno-diffusion, immunoelectrophoresis, and immunoblotting. Rabbit RSE antisera identified 2 distinct major antigenic components for untreated RSE: one related to the O-polysaccharide side chain (O-antigenic specificity), the other to the R-core. Based on a serologic cross-reactivity of R-core of RSE (Escherichia coli 0113) with the R-core of the lipopolysaccharide from E. coli 0111, the core type of E. coli 0113 was identified as coli R3. Increasing exposure of RSE to ionizing radiation progressively destroyed all antigenic reactivities; at lower doses of radiation the rate of elimination differed for the 2 antigen classes. The O-polysaccharide was more sensitive to gamma radiation than the R-core and the O-antigenicity was lost before that of the R-core. Endotoxin molecules containing incomplete R-core (radiation-induced or mutant) did not react with the RSE antiserum. Keywords: Antigenicity, Reprints. (KT/KR)
- Research Organization:
- Naval Medical Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 6074336
- Report Number(s):
- AD-A-201399/3/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
ANTIGENS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENDOTOXINS
ESCHERICHIA COLI
GAMMA RADIATION
IMMUNE SERUMS
IMMUNOLOGY
IONIZING RADIATIONS
MAMMALS
MATERIALS
MICROORGANISMS
MOLECULES
RABBITS
RADIATION EFFECTS
RADIATIONS
SENSITIVITY
TOXIC MATERIALS
TOXINS
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
ANTIGENS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENDOTOXINS
ESCHERICHIA COLI
GAMMA RADIATION
IMMUNE SERUMS
IMMUNOLOGY
IONIZING RADIATIONS
MAMMALS
MATERIALS
MICROORGANISMS
MOLECULES
RABBITS
RADIATION EFFECTS
RADIATIONS
SENSITIVITY
TOXIC MATERIALS
TOXINS
VERTEBRATES