Relation of structure to function for the US reference standard endotoxin after exposure to /sup 60/Co radiation

Journal Article · Wed Jan 01 04:00:00 EST 1986 · J. Infect. Dis.; (United States)

DOI:https://doi.org/10.1093/infdis/153.1.98· OSTI ID:5805480

Csako, G; Suba, E A; Ahlgren, A; Tsai, C M; Elin, R J

The structure and function of the highly purified US reference standard endotoxin (RSE) were studied after exposure to ionizing radiation from a /sup 60/Co source. With increasing doses of radiation, the trilaminar ribbon-like structure of untreated endotoxin exhibited focal swelling, after which only spherical particles were seen by electron microscopy. These morphological changes were paralleled by the respective loss of O-side chain repeating units and pieces of the R-core from the lipopolysaccharide molecules, as demonstrated by electrophoresis. The biologic function of the irradiated endotoxin was assessed with a variety of tests. At higher doses of radiation, a direct relation was observed between the degradation of the molecular and supramolecular structure and the loss of biologic function. At lower doses of radiation, however, there was variability among the functional assays in their rate of change with progressive irradiation of the RSE. The results suggest that the carbohydrate moiety plays an important role both in determining the supramolecular structure and in modulating certain biologic activities of bacterial endotoxins.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 5805480
• Journal Information: J. Infect. Dis.; (United States), Journal Name: J. Infect. Dis.; (United States) Vol. 1; ISSN JIDIA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOHYDRATES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
COBALT 60
COBALT ISOTOPES
DOGS
DOSE-RESPONSE RELATIONSHIPS
ENDOTOXINS
ESCHERICHIA COLI
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIPIDS
LIPOPOLYSACCHARIDES
MAMMALS
MATERIALS
MICE
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MOLECULAR STRUCTURE
MORPHOLOGY
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOISOTOPES
RODENTS
SACCHARIDES
STRUCTURE-ACTIVITY RELATIONSHIPS
TOXIC MATERIALS
TOXINS
VERTEBRATES
YEARS LIVING RADIOISOTOPES