Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Relation of structure to function for the US reference standard endotoxin after exposure to /sup 60/Co radiation, Interim report, September 1984-December 1985

Technical Report ·
OSTI ID:5803544
;
The structure and function of the highly purified U.S. reference standard endotoxin (RSE) were studied after exposure to ionizing radiation from a /sup 60/Co source. With increasing doses of radiation, the trilaminar ribbon-like structure of untreated endotoxin exhibited focal swelling, after which only spherical particles were seen by electron microscopy. These morphological changes were paralleled by the respective loss of O-side chain-repeating units and pieces of the R-core from the lipopolysaccharide molecules, as demonstrated by electrophoresis. The biologic function of the irradiated endotoxin was assessed with a variety of tests. At higher doses of radiation, a direct relation was observed between the degradation of the molecular and supramolecular structure and the loss of biologic function. At lower doses of radiation, however, there was variability among the functional assays in their rate of change with progressive irradiation of the RSE. The results suggest that the carbohydrate moiety plays an important role both in determining the supramolecular structure and in modulating certain biologic activities of bacterial endotoxins.
Research Organization:
Naval Medical Research Inst., Bethesda, MD (USA)
OSTI ID:
5803544
Report Number(s):
AD-A-165068/8/XAB; NMRI-86-1
Country of Publication:
United States
Language:
English

Similar Records

Relation of structure to function for the US reference standard endotoxin after exposure to /sup 60/Co radiation
Journal Article · Tue Dec 31 23:00:00 EST 1985 · J. Infect. Dis.; (United States) · OSTI ID:5805480
Modification of the chemical composition and structure of the US Reference Standard Endotoxin (RSE) by /sup 60/Co radiation
Journal Article · Fri Feb 28 23:00:00 EST 1986 · Radiat. Res.; (United States) · OSTI ID:5528989
Physical and biological properties of U. S. standard endotoxin EC after exposure to ionizing radiation
Journal Article · Fri Jul 01 00:00:00 EDT 1983 · Infect. Immun.; (United States) · OSTI ID:5097022

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIGENS
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL RADIATION EFFECTS
CARBOHYDRATES
COBALT 60
COBALT ISOTOPES
DISEASES
ELECTRON MICROSCOPY
ELECTROPHORESIS
ENDOTOXINS
FUNCTIONS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIPIDS
LIPOPOLYSACCHARIDES
MATERIALS
MICROSCOPY
MINUTES LIVING RADIOISOTOPES
MOLECULAR STRUCTURE
MOLECULES
MUTAGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARTICLES
POLYSACCHARIDES
RADIATION EFFECTS
RADIOISOTOPES
SACCHARIDES
SPHERES
TOXIC MATERIALS
TOXINS
YEARS LIVING RADIOISOTOPES