Characterization of a zinc blotting technique: evidence that a retroviral gag protein binds zinc

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.12.4195· OSTI ID:6922191

Schiff, L A; Nibert, M L; Fields, B N

We have characterized a simple method that uses /sup 65/ZnCl/sub 2/ to detect zinc-binding proteins that have been immobilized on nitrocellulose. Conditions have been identified that permit the detection of as little as 1 microgram of some zinc-binding proteins. The specificity of the binding is indicated by the ability of other divalent metal ions to compete with /sup 65/Zn(II) in this assay. We have used this technique to provide evidence that the nucleic acid-binding gag protein of retroviruses also binds zinc. This technique can be applied to biological mixtures of proteins and may be used in proteolytic mapping studies to identify protein fragments that have zinc-binding activity.

• Research Organization: Harvard Medical School, Boston, MA (USA)
• OSTI ID: 6922191
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:12; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
KINETICS
MEASURING METHODS
METABOLISM
METALS
MICROORGANISMS
NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
VIRUSES
ZINC
ZINC 65
ZINC ISOTOPES