Repeated doses of gamma rays induce resistance to N-methyl-N'-nitro-N-nitrosoguanidine in Chinese hamster cells

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577310· OSTI ID:6602265

Osmak, M

Chinese hamster V79 cells were preirradiated repeatedly with gamma rays and then exposed to ultraviolet (uv) light or N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The cell killing and induction of mutation at the hypoxanthine-guanine phosphoribosyltransferase locus were examined following these treatments. Cells preirradiated with multiple fractions of gamma rays exhibit the same sensitivity to uv light as the control cells with respect to cell survival and mutation induction. Following treatment with MNNG, resistance to cell killing was observed along with a decreased frequency of mutations induced. These results indicate that the progeny of cells irradiated with multiple fractions of gamma rays could display subsequent changes in sensitivity to lethal and mutagenic effects of additional treatment with DNA-damaging agents.

• Research Organization: Ruder Boskovic Institute, Zagreb (Yugoslavia)
• OSTI ID: 6602265
• Journal Information: Radiat. Res.; (United States), Vol. 115:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
MUTATIONS
RADIOINDUCTION
NITROSO COMPOUNDS
TOXICITY
CELL KILLING
DNA
FRACTIONATED IRRADIATION
GAMMA RADIATION
HAMSTERS
LUNGS
PHOSPHOTRANSFERASES
SENSITIVITY
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BODY
ELECTROMAGNETIC RADIATION
ENZYMES
IONIZING RADIATIONS
IRRADIATION
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
RODENTS
TRANSFERASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 - Chemicals Metabolism & Toxicology