Replication of simian virus 40 DNA after UV irradiation: evidence of growing fork blockage and single-stranded gaps in daughter strands

Journal Article · Tue Nov 01 04:00:00 EST 1988 · J. Virol.; (United States)

OSTI ID:6599643

Mezzina, M; Menck, C F.M.; Courtin, P; Sarasin, A

The molecular mechanisms of in vivo inhibition of mammalian DNA replication by exposure to UV light (at 254 nm) was studied in monkey and human cells infected with simian virus 40. Analysis of viral DNA by electron microscopy and sucrose gradients confirmed that the presence of UV-induced lesions severely blocks DNA synthesis, and thus the conversion of replicative intermediates (RIs) into fully replicated form I DNA is inhibited by UV irradiation. These blocked RI molecules present several special features when visualized by electron microscopy. In excision repair-proficient monkey and human cells they are composed of a double-stranded circular DNA with a double-stranded tail whose size corresponds to the average interpyrimidine dimer distance, as determined by the dimer-specific T4 endonuclease V. In excision repair-deficient human cells from patients with xeroderma pigmentosum, UV-irradiated RIs present a Carins-like structure similar to that observed for replicating molecules obtained from unirradiated infected cells. Single-stranded gaps are visualized in the replicated portions of UV-irradiated RI molecules; such regions are detected and clearly distinguishable from double-stranded DNA when probed by a specific single-stranded DNA-binding protein such as the bacteriophage T4 gene 32 product. Consistent with the presence of gaps in UV-irradiated RI molecules, single-strand-specific S1 nuclease digestion causes a shift in their sedimentation properties when analyzed in neutral sucrose gradients compared with undamaged molecules.

• Research Organization: Institut de Recherches Scientifiques sur le Cancer, Villejuif (France)
• OSTI ID: 6599643
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:11; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DISEASES
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
ENZYMES
ESTERASES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYDROLASES
MAMMALS
MAN
MICROORGANISMS
MICROSCOPY
MOLECULAR STRUCTURE
MONKEYS
NUCLEASES
NUCLEIC ACID REPLICATION
PARASITES
PHOSPHODIESTERASES
PRIMATES
RADIATION EFFECTS
RADIATIONS
SIMIAN VIRUS
SKIN DISEASES
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES
XERODERMA PIGMENTOSUM