Pathways of DNA repair in T4 phage. II. Sedimentation analysis of intracellular DNA in repair-defective mutants

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(76)90069-6· OSTI ID:7325867

Wakem, L P; Ebisuzaki, K

The function of various pathways of repair was assessed by sucrose gradient centrifugation of uv-irradiated intracellular T4 DNA. Mutation in the ..nu.. gene inhibits the excision repair pathway and results in a slower endonucleolytic reaction, a delay in ''joining'' of fragments and a decreased rate of DNA replication following uv irradiation. Mutations in the x or y genes inhibit an alternate pathway and result in the failure to ''join'' single-strand breaks after irradiation. In the absence of uv irradiation, the x and y mutations lead to the accumulation of DNA with single-strand breaks and a decrease in the amount of concatemers. We postulate that the x and y genes are involved in a ''joining'' reaction involving DNA fragments.

• Research Organization: Univ. of Western Ontario, London
• OSTI ID: 7325867
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 73; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
IRRADIATION
MICROORGANISMS
MUTATIONS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATIONS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VIRUSES