Replicate intermediates in ultraviolet-irradiated Simian virus 40

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1984

OSTI ID:5586292

Clark, J M

Simian virus 40 (SV40) was used as a probe to study the replication of UV-damaged DNA in mammalian cells. Viral DNA replication in infected monkey kidney cells was synchronized using a mutant of SV40 (tsA58) temperature-sensitive for the initiation of DNA synthesis, and aphidicolin to maintain replication synchrony at the permissive temperature by temporarily blocking DNA synthesis while permitting pre-replicative events to occur. After removal of the drug, the infected cells were irradiated at 100 J/m/sup 2/ (254 nm) to produce 6-7 pyrimidine dimers per SV40 genome, and returned to the restrictive temperature to present reinitiation of replication from the SV40 origin. The size distribution of daughter strands in replicative intermediates (RI) isolated shortly after irradiation was skewed towards lengths less than the interdimer spacing. Our results are consistent with the hypothesis that both DNA strand exchange and trans-dimer synthesis participate in the generation of supercoiled Form I DNA from UV-damaged SV40 replicative intermediates.

• Research Organization: Stanford Univ., CA (USA)
• OSTI ID: 5586292
• Country of Publication: United States
• Language: English

Related Subjects

560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CULTURES
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
EXTREME ULTRAVIOLET RADIATION
HETEROCYCLIC COMPOUNDS
KIDNEYS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
SIMIAN VIRUS
SYNCHRONOUS CULTURES
THYMIDINE
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VIRUSES