Survival of spermatogonial stem cells in the CBA mouse after combined exposure to 1-MeV fission neutrons and hydroxyurea

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575436· OSTI ID:6596063

de Ruiter-Bootsma, A L ^[1]; Davids, J A.G.

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1. State Univ., Medical School, Utrecht, Netherlands

Single-neutron dose response studies have demonstrated the presence in the CBA mouse testis of about 2000 radioresistant (D/sub 0/ = 80 rad) colony-forming stem cells. After surviving a dose of 150-rad neutrons, these cells rapidly increase their radiosensitivity. The second dose survival showed a cyclic pattern: a decrease to very low levels during the first 16 h after the initial dose, a rise to higher levels from 40 h on, and a second decrease starting at 56 h. To investigate whether the fluctuations in stem-cell survival are correlated with a synchronized progress of surviving stem cells through their first postirradiation cell cycle, we replaced the second neutron dose by an injection with the S-phase-specific cytotoxic agent hydroxyures (HU). The pattern of stem-cell survival found confirmed the synchronization. HU given before neutron irradiation resulted in a sharp decline of stem-cell survival down to 3% of the level after irradiation alone when the treatment interval was prolonged to 16 h. This indicates that the great majority of the potential neutron survivors were in an S phase of their cell cycle 16 h before irradiation. It may not be excluded, however, that the great amount of stem-cell killing in the HU-neutron experiments was caused by a triggering by HU of radioresistant slow-cycling cells into rapid proliferation, accompanied by a sharp increase of their radiosensitivity.

• OSTI ID: 6596063
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 85:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
CELL KILLING
ELEMENTARY PARTICLES
FERMIONS
FISSION NEUTRONS
GAMETES
GERM CELLS
HADRONS
HYDROXY COMPOUNDS
HYDROXYUREA
MAMMALS
MICE
NEUTRONS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
SPERMATOGONIA
STEM CELLS
SURVIVAL CURVES
VERTEBRATES