Strain differences in the response of mouse testicular stem cells to fractionated radiation

Journal Article · Thu Mar 01 04:00:00 EST 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576138· OSTI ID:7133603

Meistrich, M L ^[1]; Finch, M; Lu, C C; de Ruiter-Bootsma, A L; de Rooij, D G; Davids, J A.G.

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1. Univ. of Texas, Houston

The survival of spermatogonial stem cells in CBA and C3H mice after single and split-dose (24-hr interval) irradiation with fission neutrons and gamma rays was compared. The first doses of the fractionated regimes were either 150 rad (neutrons) or 600 rad (gamma). For both strains the neutron survival curves were exponential. The D/sub 0/ value of stem cells in CBA decreased from 83 to 25 rad upon fractionation; that of C3H stem cells decreased only from 54 to 36 rad. The survival curves for gamma irradiation, which all showed shoulders, indicated that C3H stem cells had larger repair capacities than CBA stem cells. However, the most striking difference between the two strains in response to gamma radiation was in the slopes of the second-dose curves. Whereas C3H stem cells showed a small increase of the D/sub 0/ upon fractionation (from 196 to 218 rad), CBA stem cells showed a marked decrease (from 243 to 148 rad). The decreases in D/sub 0/ upon fractionation, observed in both strains with neutron irradiation and also with gamma irradiation in CBA, are most likely the result of recruitment or progression of radioresistant survivors to a more sensitive state of proliferation or cell cycle phase. It may be that the survivng stem cells in C3H mice are recruited less rapidly and synchronously into active cycle than in CBA mice. Thus, it appears that the strain differences may be quantitative, rather than qualitative.

• OSTI ID: 7133603
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 97:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CYCLE
CELL KILLING
DATA
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
EXPERIMENTAL DATA
FERMIONS
FISSION NEUTRONS
FRACTIONATED IRRADIATION
GAMETES
GAMMA RADIATION
GERM CELLS
GONADS
HADRONS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
MALE GENITALS
MAMMALS
MICE
NEUTRONS
NUCLEONS
NUMERICAL DATA
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RBE
RODENTS
SOMATIC CELLS
SPERMATOGONIA
STEM CELLS
SURVIVAL CURVES
TESTES
VERTEBRATES