Survival of mouse testicular stem cells after. gamma. or neutron irradiation

Journal Article · Sat Mar 01 04:00:00 EST 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575199· OSTI ID:5323192

Lu, C C ^[1]; Meistrich, M L; Thames, Jr, H D

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1. Univ. of Texas System Cancer Center, Houston

The survival of mouse testicular stem cells after ..gamma.. or neutron irradiation was measured by counts of repopulated tubular cross sections and by the numbers of differentiated spermatogenic cells produced. The numbers of such cells were determined either by sperm head counts of the X-isozyme of lactate dehydrogenase enzyme levels. Qualitatively similar results were obtained with all three assays. The results have confirmed that, with C3H mice, stem-cell survival is higher when the ..gamma..-radiation dose is fractionated by a 24-h interval. Single-dose ..gamma..-radiaton survival curves for the stem cell had large shoulders and also showed the presence of a radioresistant subpopulation which predominated after doses greater than 600 rad. Part of the shoulder must have resulted from repair of sublethal damage since neutron irradiation produced survival curves with smaller shoulders. The relative biological effectiveness for stem-cell killing for these neutrons (mean energy, 22 MeV) varied from about 2.9 at 10 rad of ..gamma.. radiation to 2.2 at 600 rad.

• OSTI ID: 5323192
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 81:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

551000 -- Physiological Systems
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560152 -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL KILLING
DATA
DATA FORMS
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FRACTIONATED IRRADIATION
GAMETOGENESIS
GAMMA RADIATION
GONADS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
MALE GENITALS
MAMMALS
MEDICINE
MICE
NEUTRON THERAPY
NUCLEAR MEDICINE
NUMERICAL DATA
ORGANS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOLOGY
RADIOSENSITIVITY
RADIOTHERAPY
RBE
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
SPERMATOGENESIS
STEM CELLS
SURVIVAL CURVES
TESTES
THERAPY
VERTEBRATES